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Isolation of Inner Cell Mass for the Establishment of Human Embryonic Stem Cell (hESC) Lines
Isolation of Inner Cell Mass for the Establishment of Human Embryonic Stem Cell (hESC) Lines
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机译:分离内部细胞团以建立人类胚胎干细胞(hESC)系
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摘要
A method of establishing a cell line from the inner cell mass of a blastocyst, comprising: isolating a blastocyst having a zona pellucida, a trophectoderm, and an inner cell mass; creating an aperture in the blastocyst by laser ablation; isolating cells from the inner cell mass from the blastocyst through the aperture; and culturing the cells to establish a cell line, wherein the cells are cultured under feeder free conditions.
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