Methods To Identify, Prepare, And Use Naive T Cell Recent Thymic Emigrants

摘要

A method of purifying a subpopulation of naive T cells that have recently emigrated from the thymus, comprising (a) contacting a biological sample susceptible of containing T cells with at least four different ligands, namely ligands directed to the markers CD3, CD4, CD45RA, and CD31; (b) sorting the cells so as to recover T cells having a phenotype comprising at least the following four markers CD3⁺, CD4⁺, CD45RA⁺ and CD31^hi, whereby a subpopulation of naive T cells is purified.