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Expression of Streptomyces subtilism inhibitor (SSI) proteins in Bacillus and Streptomyces sp.
Expression of Streptomyces subtilism inhibitor (SSI) proteins in Bacillus and Streptomyces sp.
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机译:链霉菌枯草杆菌抑制剂(SSI)蛋白在芽孢杆菌和链霉菌种中的表达。
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摘要
Streptomyces and Bacillus host cells comprising a recombinant nucleic acid encoding a fusion protein containing a signal sequence and a Streptomyces subtilisin inhibitor (SSI) protein are provided, as well as methods of producing SSI protein using those cells. In certain embodiments, the host cell is a Streptomyces host cell and the signal sequence is a celA signal sequence. In other embodiments, the host cell is a Bacillus host cell has a genome that contains inactivated protease genes.
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