Methods of Validating Target for Modulating Insulin Action, Screening for Modulators of Insulin Action and Therapeutic Uses Thereof

摘要

The present invention provides several assay formats for determining the effect of compounds on one or more metabolism-associated phenotypes in a cell, tissue or animal. The assay formats are based upon the finding that Cbl (e.g., the protooncogene c-Cbl) is an upstream regulator of AMPK activity and ACC activity in muscle, and that this regulatory pathway modifies whole body energy expenditure and whole body insulin sensitivity.