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Large-Scale Production of Recombinant Transmembrane and Cytosolic Proteins
Large-Scale Production of Recombinant Transmembrane and Cytosolic Proteins
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机译:大规模生产重组跨膜和胞质蛋白
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摘要
The present invention provides a method for producing large quantities of transmembrane and cytosolic proteins in a mammalian muscle cell. The method involves transfecting skeletal muscle cells in vivo with nucleic acids encoding the proteins by electroporation. The invention results in production of the desired protein on the order of about 2-3 magnitudes more as compared to standard methods, allowing for various biological uses including purification and crystallization.
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