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Novel calcium-independent phospholipases A2, genes thereof and promoter of the same
Novel calcium-independent phospholipases A2, genes thereof and promoter of the same
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机译:新型的非钙依赖性磷脂酶A2,其基因及其启动子
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摘要
Novel calcium-independent phospholipases A2; genes encoding the same; an antibody against them; an inherent promoter or a regulator gene which comprises a base sequence occurring in intron and inducing site-specific expression in response to an external stimulus; a method of expressing a target protein in response to an external stimulus; and an organism having this gene transferred thereinto. Novel calcium-independent phospholipases A2 having an amino acid sequence represented by SEQ ID NO: 1, 3 or 5 or an amino acid sequence derived from such an amino acid sequence by the substitution, deletion or addition of one or more amino acids; a gene having a base sequence occurring in an intron and being capable of initiating RNA transcription due to an external stimulus such as a stimulus with kainic acid or an electrical stimulus; a method of regulating expression by using the gene; and an organism having the gene transferred thereinto.
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机译:新型非钙依赖性磷脂酶A 2 Sub>;编码相同的基因;针对他们的抗体;固有的启动子或调节基因,其包含内含子中出现的碱基序列,并响应于外部刺激而诱导位点特异性表达;一种响应外界刺激而表达靶蛋白的方法;以及将该基因转移到其中的生物。具有由SEQ ID NO:1、3或5表示的氨基酸序列或通过取代,缺失或添加而衍生自该氨基酸序列的氨基酸序列的新颖的非钙依赖性磷脂酶A 2 Sub>一种或多种氨基酸;具有在内含子中出现的碱基序列的基因,并且由于诸如卡因酸或电刺激的外部刺激而能够启动RNA转录的基因;利用该基因调节表达的方法;以及将基因转移到其中的生物。
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