首页> 外国专利> In vitro resveratrol-rich callus tissues derived from vitis thunbergii Sieb.et Zucc and method for producing the same

In vitro resveratrol-rich callus tissues derived from vitis thunbergii Sieb.et Zucc and method for producing the same

机译：源自黑葡萄（Vitis thunbergii Sieb.et Zucc）的富含白藜芦醇的体外愈伤组织及其制备方法

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摘要

The present invention provides an in vitro resveratrol-rich callus tissue of Vitis thunbergii Sieb. et Zucc. which is a callus tissue developed from a tissue culture system containing one or more plant growth regulators (PGRs) and cultured from a stem or a petiole tissue explant of a wild type of V. thunbergii or a cultivated plantlet of V. thunbergii. The cultivated plantlet is in turn derived from a shoot of the wild type of V. thunbergii cultivated in a plantlet culture system containing no PGRs. The in vitro resveratrol-rich callus tissue of V. thunbergii is characterized by its containing at least about 1,000 to 10,000 mg/kg of dried weight of resveratrol, predominantly in the form of trans-resveratrol and/or resveratrol-O-glucoside, and being ready for harvest or subculture in about 30 days. The present invention further provides a method for cultivating the in vitro resveratrol-rich callus tissue, a method for extracting the resveratrol from the in vitro resveratrol-rich callus tissue, and a method for determining the resveratrol amount in the in vitro resveratrol-rich callus tissue by HPLC.

机译：本发明提供了Vitis thunbergii Sieb的富含白藜芦醇的体外愈伤组织。 et Zucc。愈伤组织是从包含一种或多种植物生长调节剂（PGR）的组织培养系统中发育而来的愈伤组织，并从野生型的黑线菌（V. thunbergii）或栽培的黑线菌（V. thunbergii）的茎或叶柄组织外植体培养而来。栽培的小植物继而来自在不包含PGR的小植物培养系统中栽培的野生型黑松菌的芽。雷氏弧菌的富含白藜芦醇的体外愈伤组织的特征在于，其含有至少约1,000至10,000 mg / kg干重的白藜芦醇，主要为反式白藜芦醇和/或白藜芦醇-O-葡萄糖苷形式，和在30天内准备好收获或传代培养。本发明进一步提供了一种用于培养所述富含白藜芦醇的离体愈伤组织的方法，一种用于从所述富含白藜芦醇的离体愈伤组织中提取白藜芦醇的方法，以及一种用于测定所述富含白藜芦醇的离体愈伤组织中白藜芦醇含量的方法。 HPLC检测组织。

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公开/公告号US2008160560A1

专利类型
公开/公告日2008-07-03

原文格式PDF
申请/专利权人 CHIN-WEN HO;HSIEN-SHEN KUO;
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申请/专利号US20070822117
发明设计人 HSIEN-SHEN KUO;CHIN-WEN HO;
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申请日2007-07-02
分类号C12P7/22;C12N5/04;C12Q1/02;
国家 US
入库时间 2022-08-21 20:12:49

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