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Method of Inducing Genome Reorganization Via Intracellular Activation of Thermostable Multifrequency Dna-Cleaving Enzyme
Method of Inducing Genome Reorganization Via Intracellular Activation of Thermostable Multifrequency Dna-Cleaving Enzyme
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机译:通过细胞内激活热稳定的多频率Dna裂解酶诱导基因组重组的方法
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摘要
The present invention relates to a method for increasing genetic recombination frequency in a genomic DNA and a method for inducing genome rearrangement. Specifically, according to the present invention there are provided: the method for increasing genetic recombination frequency in a cell in which genetic recombination takes place at any sites in the genome, comprising causing a restriction enzyme to be expressed in the cell, inducing transient activation of the restriction enzyme, and then introducing 2 or more double strand cleavages into any genomic DNA of the cell, so as to increase the genetic recombination frequency; the method for inducing genome rearrangement through the use of the above method; and cells each prepared through the use of the above 2 methods.
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