The test of release of fluorescence with respect to the protease activity of the botulism toxin in GFP - snap25

摘要

The test of release of fluorescence with respect to the protease activity of the botulism toxin in GFP - snap25.The present invention relates to a molecule of nucleic acid containing a nucleotide sequence encoding a substrate snap 25 which includes (1) a green fluorescent protein; (II) a first partner of a dual affinity; and (III) a portion of the snap 25 which includes a SEQ. u00fcu00eancia recognition BoNT / A, BoNT / C1 or BoNT / E containing a cleavage site.In the place of cleavage intervenes between the green fluorescent protein, and the first partner of dual affinity.It is also provided here in this patent application, one molecule of nucleic acid containing a nucleotide sequence encoding a substrate of toxin target that includes (1) a fluorescent protein, (II) a first partner of a dual affinity; and (III) a sequence of reconhe Cement of toxin of the clostru00eddico containing a cleavage site.In the place of cleavage intervenes between the fluorescent protein and the first partner of dual affinity.