GENETICALLY ENGINEERED CELL CULTURE ADAPTED INFECTIONS BURSAL DISEASES VIRUS (IBDV) MUTANTS

摘要

The present invention relates to a method for the adaptation of infectious bursal disease viruses (IBDV) to growth in CEF cell culture. Changing the codons for amino acid residues 253 (Gln) and 284 (Ala) to 253 (His) and 284 (Thr) allowed bursa adapted Classical and Variant-E IBDV to grow in CEF cell culture. For GLS IBDV only a change of the codon for amino acid residue 284 was necessary.