METHOD FOR OBTAINING IN VITRO RECOMBINED POLYNUCLEOTIDE SEQUENCES, SEQUENCE BANKS AND RESULTING SEQUENCES

摘要

The invention concerns a method for producing in vitro recombined polynucleotide sequences from a bank of polynucleotide sequences, characterised in that it comprises the following steps: fragmenting an initial bank of double-strand polynucleotide sequences; (b) denaturation of the fragments resulting from step (a) optionally in the presence of one or several assembling matrix or matrices; (c) hybridising said fragments with one or several of the assembling matrix or matrices if the latter is/are not present in step (b); (d) ligation of said fragments; (e) optionally cloning said recombined polynucleotide sequences having advantageous properties with respect to one or several reference sequences.