METHOD FOR OBTAINING IN VITRO RECOMBINED POLYNUCLEOTIDE SEQUENCES, SEQUENCE BANKS AND RESULTING SEQUENCES

摘要

the present invention is directed to a process for in vitro production ofrecombinant polynucleotide sequences from a library of sequencespolynucleotide, characterised in that it comprises the following steps: (a)the fragmentation of a first polynucleotide sequence double bankblades; and (b) the distortion of the fragments derived from the step (a), orin the presence of one or more matrix assembly (s); (c) hybridizationthose fragments with one or more template (s) for assembly if the (s) -is (are) not present (s) in the step (b); (d) the obligation of thethe fragments; and (e) a polynucleotide sequences or cloningrecombinant polynucleotide sequences and the recombinant selection andthe selection of polynucleotide sequences with different propertiesbenefits in relation to one or more reference sequences.