A NUCLEIC ACID WHICH ENCODES AN ENVIRONMENTALLY STABLE FLUORESCENT INDICATOR AND ENVIRONMENTALLY STABLE SENSORS INCORPORATING THE SAME

摘要

Environmentally stable biosensors are disclosed, which comprise a ligand binding domain from a thermophilic organism conjugated to donor and fluorescent moieties that permit detection and measurement of Fluorescence Resonance Energy Transfer upon ligand binding. Such biosensors demonstrate enhanced acid-, thermal- and chemical stability as compared to sensors constructed using protein domains from mesophilic organisms.