A PROCESS FOR THE PREPARATION OF A NEW HYBRIDOMA CLONE SECRETING MONOCLONAL ANTIBODY SPECIFIC TO PLASMODIAL LACTATE DEHYDROGENASE

摘要

a. immunization of BALB/c mice with purified malaria parasite lactate dehydrogenase; b. fusion of spleen cells from immunized mouse;With Sp2/0 myeloma cells in presence of polyethylene glycol and selection of hybrido¬ ma s; c. testing of culture supernatants in Enzyme linked; lmmunosorbeht assay for antibodies to malaria parasite lactate dehydrogenase using purified parasite LDH as antigen; d. selection of hybridoma clone secreting monoclonal antibody showing specificity to plasmodial 'LDH and detecting; the; parasite LDH in blood isamples, binding to loop region of'parasite LDH aftd