A PROCESS FOR THE ISOLATION OF A MAJOR HARMFUL OXIDANT FORM CIGARETTE SMOKE,P-BENZOSEMIQUINONE

摘要

The components of cigarette smoke, known until now, do not explain the overwhelming hazardous effects of smoking, this invention describes the isolation, identification and procedures for determination of the structure, properties and assay of a relatively stable major harmful oxidant (cs-oxidant) present in the cigarette smoke, the content of which is about 190±10 µg per cigarette, the cs-oxidant alone almost quantitatively accounts for the oxidative damage of proteins produced by the aqueous extract of whole cigarette smoke, the cs-oxidant is also responsible for the oxidative damage of DNA, since the cs-oxidant is relatively stable, it further explains the deleterious effects of the sidestream smoke and passive smoking, a number of chemical compounds / agents including vitamin C have been found to prevent the cs-oxidant induced protein oxidation in vitro.