MASS MULTIPLICATION OF PSEUDOMONAS FLUORESCENS ON COW DUNG

摘要

This invention relates to a process for mass multiplication of Pseudomonas fluorescens on cow dung comprising the steps of air drying of cow dung by spreading to form layer of 2.5 thickness under open shade for 5 to 7 days, maintaining the moisture content to 40 % by weight of air dried cow dung by adding water, maintaining the pH of cow dung between 7 to 7.5, autoclaving the cow dung of step b) at 15 Ibs for 30 minutes, 2 ml to 5 ml suspension of Pseudomonas fluorescens PBAP-27 talc-based formulation (@ lg/ 100 ml; approx. population 106 cfu/ml) was added and mixed thoroughly under aseptic condition, after 2 weeks of incubating at 30±2 °C the Pseudomonas fluorescens multiplied to 1014 cfu/g air dried cow dung, Pseudomonas fluorescens colonized cow dung was dried, powdered and formulated with talc containing carboxymethyl cellulose (@1% w/w) or mineral oil to obtain final concentration of £ 2 x 108 cfu/g or per ml formulation. (MONA SAINI) OF L.S.DAVAR & CO., APPLICANTS ATTORNEY.