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A METHOD FOR CULTURING A MICROORGANISM HAVING A METHANOL METABOLIC PATHWAY
A METHOD FOR CULTURING A MICROORGANISM HAVING A METHANOL METABOLIC PATHWAY
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机译:培养具有甲醇代谢途径的微生物的方法
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摘要
A method for culturing a microorganism having methanol metabolic pathway and into said microorganism an expression unit has been introduced wherein said expression unit comprises a target gene which is controlled by a promoter that can be derepressed by starvation on glycerol and induced with methanol or mixed feed of methanol and glycerol and said method comprising of; culturing the microorganism in a nutrient medium characterized in that maintaining the dissolved oxygen level between 50 % and 95% of saturation level in the presence of glycerol in growth phase; feeding a limiting amount of glycerol for derepression of the target gene wherein the dissolved oxygen fluctuates between 0% to 80%; inducing the target gene by feeding methanol or methanol/glycerol feed at a rate greater than the maximum methanol consumption rate of the microorganism wherein the dissolved oxygen level fluctuates synchronously between 0% and 100% with methanol addition.
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