METHOD OF GENERATING A DIFFERENTIATED NEURAL CELL POPULATION FROM MAMMALIAN PLURIPOTENT STEM CELLS FOR TREATING NEURODEGENERATIVE DISEASES

摘要

A method of generating a differentiated neural cell population from mammalian pluripotent stem cells for treating neurodegenerative diseases comprising the following steps: (a) expanding a culture ofpluripotent stem cells; (b) culturing the pluripotent stem cells to select for neuroprogenitor cells that are positive for nestin; (c) expanding the nestin-positive neuroprogenitor cells; and (d) differentiating the nestin-positive cells to generate a differentiated cell population by culturing the cells in a differentiation media which comprises cytosine P-d-Arabino ftiranoside (Ara-C).