A NOVEL MOLECULARLY IMPRINTED POLYMER (MIP)-IMMOBILISED SILICA GEL SORBENT PREPARATION AND A PROCESS FOR DETERMINATION OF BARBITURIC ACID USING THE SAME MIP-BASED SORBENT

摘要

The present invention refers to a novei molecularly imprinte to lamer (MIP) obtainable by polymerisation of monomers in the presence of a template molecule barbituric acid (BA). The obtained linear MIP may be used for selective separation of the template analyte from blood samples in aqueous samples, effluents, pharmaceutical preparations in the presence of other structurally related interferents. Present invention further led refers to the preparation of new a silica gel bound molecularly imprinted polymer-based sorbent of column chromatography for the selective recognition of barbituric acid (BA). The optimised operaţional conditions for 100% retention of the barbituric acid on a chromatographic column (13.0 x 1.4 cm), packed with adsorbent bed 1.0 g, is realised at pH 7.0 and a flow rate of 5.0 mL min"1. The MlP-based adsorbent can be instantaneously regenerated for reuse after washing with hoţ water within 15 min.