首页>
外国专利>
DETECTION OF VERY VIRULENT INFECTIOUS BURSAL DISEASE VIRUS.
DETECTION OF VERY VIRULENT INFECTIOUS BURSAL DISEASE VIRUS.
展开▼
机译:检测非常致命的传染性法氏囊病病毒。
展开▼
页面导航
摘要
著录项
相似文献
摘要
Methods of identifying animals infected with a vvIBDV are provided. The methods comprise contacting a nucleic acid sample obtained from the animal or a nucleic acid product obtained by amplifying RNA obtained from the animal with one or more probe pairs, each of which comprises a mutation probe and an anchor probe, and then determining the melting temperature of any hybridization complex that is formed when the one or more probe pairs hybridize with a nucleic acid in the sample. Such determination is made using FRET analysis. In one embodiment the mutation probe comprises a sequence identical to a first mutated target sequence of SEQ ID NO: 1 in which the cytosine at position 827 is substituted with a thymidine, the cytosine at position 830 is substituted with a thymidine, and the thymidine at position 833 is substituted with a cytosine, or the reverse complement thereof. In another embodiment, the mutation probe comprises a sequence identical to a second mutated target sequence of SEQ ID NO: 1 in which the guanine at position 897 is substituted with an adrenine, the cytosine at position 905 is substituted with a thymidine, and the cytosine at position 908 is substituted with an thymidine. Also, provided herein are kits containing nucleotide probe pairs that can be used in the present methods.
展开▼
机译:提供了鉴定被vvIBDV感染的动物的方法。该方法包括使从动物获得的核酸样品或通过将从动物获得的RNA扩增的核酸产物与一个或多个探针对接触,所述探针对各自包含突变探针和锚定探针,然后确定解链温度当一个或多个探针对与样品中的核酸杂交时形成的任何杂交复合物。这种确定是使用FRET分析进行的。在一个实施方案中,突变探针包含与SEQ ID NO:1的第一突变靶序列相同的序列,其中827位的胞嘧啶被胸苷取代,830位的胞嘧啶被胸苷取代,而胸腺嘧啶在833位被胞嘧啶或其反向互补物取代。在另一个实施方案中,突变探针包含与SEQ ID NO:1的第二突变靶序列相同的序列,其中897位上的鸟嘌呤被肾上腺素取代,905位上的胞嘧啶被胸苷取代,而胞嘧啶在位置908处的胸苷被胸苷取代。另外,本文提供了可用于本方法的包含核苷酸探针对的试剂盒。
展开▼