Gene silencing by double-stranded RNA in plants using a type 3 Pol III promoter

摘要

Disclosed are methods and means to increase the efficiency of gene silencing when using dsRNA sequences which have a stem length shorther than about 200 basepairs by providing chimeric genes encoding such dsRNA sequences with a promoter recognized by DNA dependent RNA polymerase III comprising all cis-acting promoter elements which interact with DNA dependent RNA polymerase III.