Flow cytometer for high purity X-chromosome bearing and Y-chromosome bearing populations of spermatozoa

摘要

Disclosed is a particle differentiation apparatus, comprising: a) a fluid stream; b) intact live sperm cells introduced into said fluid stream; c) a nozzle having a orifice through which said fluid stream exits; d) an oscillator responsive to said fluid stream; and e) droplets breaking off from said fluid stream, wherein a plurality of said droplets entrain said intact live sperm cells, and wherein said droplets have sufficient size to entirely encapsulate one of said live sperm cells.