首页>
外国专利>
Method of determining epidermal growth factor receptor gene expression in a parafin embedded tiasue sample and correlation of levels thereof with survival rates
Method of determining epidermal growth factor receptor gene expression in a parafin embedded tiasue sample and correlation of levels thereof with survival rates
展开▼
机译:确定石蜡包埋的组织样品中表皮生长因子受体基因表达的方法及其水平与存活率的相关性
展开▼
页面导航
摘要
著录项
相似文献
摘要
Disclosed is a method for the determination of the level of Epidermal Growth Factor receptor (EGFR) expression in a fixed paraffin embedded tissue sample comprising, deprarfinising the tissue sample, isolating the mRNA from the sample in the presence of a chaotropic agent, subjecting the mRNA to amplification using oligonucleotide primers that hybridise under stringent conditions to a region of the EGFR gene to obtain an amplified sample then determining the quantity of the EGFR mRNA compared to an internal control gene, which can be beta-actin.
展开▼