a heat stable phytase thermoactive, with good stability during storage can be incorporated as an additive in animal feed, and a sequence coding for this enzyme nucleotidique

摘要

the present invention relates to the screening of phytase activity (phyc us417) thermoactive and thermostable strain bacillus subtilis us417 secreted by newly isolated, purification and physicochemical characterization of the enzyme, cloning and sequence determination the nucleotide and amino acid of said phytase activity.this enzyme has the features such as thermoactivitu00e9, thermostability, good stability during storage without the addition of stabilizers and / or conservative and strong specificity for phytic acid, which is a factor antinutritionn in animal feed and cereal based foods.in fact, it is a chelating agent which forms a complex with the major food of important divalent cations (ca2 +, mg2 +, zn2 +, fe2 +, reducing their bioavailability (harland and oberleas, 1999; sebastian et al., 1998). in addition, this enzyme is active and stable over a wide ph range, which makes it attractive for applications in animal feed.