Method for prediction of remodeling of bronchi on background of chronic and recurring bronchitis

摘要

Method for prediction of remodeling of bronchi on background of chronic and recurring bronchitis includes brash-biopsy of bronchi mucous membrane, immuno-cytochemical investigation of analizate, evaluation of data and formation of conclusion. Additionally analyzate undergoes separate titration with use of monoclonal antibodies TGF-, 3412, -67 and glasses, with incubation at 25-30 ° during 30 minutes, one washet titers, with covering in sequence with layers of diaminobenzidine, gematoxyline, one determines levels of expression of monoclonal anti-bodies in each of titers, one evaluated reactions of interaction of antibodies and anti-genes, and at formation of conclusion, on basis of dependence of fibrosis of bronchial wall on working-out proliferative resource and cell metaplasia of bronchial epithelium one predicts high risk of remodeling pathogenesis, if values of expressions TGF- and 3412 or -67 exceed levels of expressions of respective normals.