Method for the analysis of AFLP reaction mixtures using techniques of primer extension.

摘要

A method for analyzing a mixture of writing AFLP by the presence or absence of fragments target restriction in a mixture of restriction fragments, using oligonucleotide sequence that are specific each for only a target restriction fragment, said method comprising the steps of: a) contacting a mixture of AFLP reaction with at least three oligonucleotide sequences under hybridizing, such that when the fragments target restriction are present, hybrids are formed between the restriction fragments objective and sequences oligonucleotide, such that the resulting hybrids have at least one unpaired nucleotide of the target restriction fragment directly adjacent to the 3 '' of the oligonucleotide sequence; b) adding at least one labeled nucleotide or nucleotide analogue to the mixture resulting from step a) under conditions suitable for extension of the oligonucleotides conditions so that when said hybrids are present the oligonucleotides are extended with the labeled oligonucleotide or the nucleotide analog; c) detecting the presence or absence of any hybrid with the labeled nucleotide added in or nucleotide analog, and / or any oligonucleotide sequence with an added labeled nucleotide analogue or nucleotide.