Method for monitoring of an analyte using an electrochemical biosensor that can be switched by applying an electric potential.

摘要

A method for monitoring analyte concentration in a biological fluid using a sensor, and this method comprises the steps of: providing a sensor comprising a retention volume of a hydrophilic medium, forming this volume for retaining an amount analyte is proportional to the concentration of analyte in a biological fluid in contact with the sensor, an enclosure for said volume of analyte retention, the enclosure defined at least in part by a wall comprising an area of ​​a membrane being permeable to the analyte (20, 220) with a first side in contact with the hydrophilic medium, an electrode in contact with the hydrophilic medium, a redox enzyme in contact with the medium, and an electron transfer mediator to facilitate electron transfer between the enzyme and the electrodes being substantially incapable of transferring electrons to or enzyme from c omponents of different biological fluid to said analyte, contacting the biological fluid with the sensor and at intervals applying a potential to sufficient electrode to oxidize the electron mediator and sensing current through said electrode as a function of the length of applied potential, maintaining the oxidation potential of the mediator applied at least for a sufficient period of time to determine the rate of change of current over time at the electrode, and correlating the current flow with the current flow of concentrations known of said analyte in the retention means, wherein the method further comprises the step of applying to the electrode sufficient to reduce the oxidized form electron mediator potential.