cell lines stably transfected, usable for identifying components that affect mRNA stability.

摘要

A stably transfected cell line used for screening of compounds for treating disease interest comprising: (i) An expression system reporter gene DNA consists of a reporter gene encoding a first protein which gives a detectable signal, a sequence 5''UTR and 3''UTR sequence, wherein said sequence and said 3''UTR 5''UTR sequence comprise appropriate elements for controlling expression, and DNA sequence consisting of 20-100 nucleotides They contain mRNA instability sequence derived from a gene sequence related to said reporter gene different from said disease of interest and wherein said DNA sequence is inserted into the sequence 3''UTR reporter gene; and (ii) a control of the expression of reporter gene DNA including a reporter gene, encoding a second protein which gives a detectable signal, a sequence 5''UTR and 3''UTR sequence, wherein said sequence 5 '' UTR and dichasecuencia 3''UTR contain appropriate elements for controlling expression, but lacks any functional mRNA instability sequence, and wherein the cell line derived from the native cell type in which said instability sequence is produced mRNA. 1