squalene synthase gene (SQS).

摘要

A recombinant organism whose gene expression of squalene synthase is reduced compared to the host organism, which is capable of producing carotenoids in an enhanced relative to the host organism, characterized in level because the recombinant organism is a fungal cell containing an antisense polynucleotide against a nucleic acid molecule selected from the group consisting of: (a) nucleic acid molecules encoding at least the mature form of the polypeptide depicted in SEQ ID NO: 3; (B) nucleic acid molecules comprising the coding sequence as displayed in SEQ ID NO: 2; (C) nucleic acid molecules whose nucleotide sequence is degenerate as a result of the genetic code to a nucleotide sequence of (a) or (b); (E) nucleic acid molecules encoding a polypeptide derived from the polypeptide whose sequence has an identity of 51.3% or more with the amino acid sequence of the polypeptide encoded by a nucleic acid molecule of (a) or (b); (G) nucleic acid molecules comprising a polynucleotide having a sequence of a nucleic acid molecule amplified from a nucleic acid library of Phaffia using the primers depicted in SEQ ID NO: 4, 5 and 6; (J) nucleic acid molecules encoding a polypeptide having squalene synthase activity, wherein said polypeptide is recognized by antibodies that have been raised against a polypeptide encoded by a nucleic acid molecule of any one (a), (b) , (c) and (g); (K) nucleic acid molecules obtainable by screening an appropriate library under stringent conditions with a probe having the sequence of the nucleic acid molecule of any one of (a), (b), (c), (e ), (g) and (j), and encoding a polypeptide having squalene synthase activity.