ANTIBODIES SPECIFIC FOR A HAEMOSTATIC PROTEIN, THEIR USE FOR ISOLATING INTACT PROTEIN, HAEMOSTATIC COMPOSITIONS DEVOID OF PROTEOLYTIC CLEAVAGE PRODUCTS OF THE PROTEIN

摘要

Methods for the generation of Ca2+ -independent antibodies against bloodcoagulation factors, employing an antibodyselection strategy based on small peptides that are comprising targetsequences for limited proteolysis. These antibodies,which substantially distinguish between intact and cleaved species of saidhaemostatic protein, provide novel tools for theisolation of intact haemostatic proteins. By virtue of the absence of cleavageproducts that usually are associated withside-effects or reduced efficacy, these intact proteins may serve as improvedagents in therapeutic compositions for the treatmentof haemostatic disorders.