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USE OF RESISTIVE-PULSE SENSING WITH SUBMICROMETER PORES OR NANOPORES FOR THE DETECTION OF THE ASSEMBLY OF SUBMICROMETER OR NANOMETER SIZED OBJECTS
USE OF RESISTIVE-PULSE SENSING WITH SUBMICROMETER PORES OR NANOPORES FOR THE DETECTION OF THE ASSEMBLY OF SUBMICROMETER OR NANOMETER SIZED OBJECTS
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机译:带有亚微孔或纳米孔的电阻脉冲感测器用于检测亚微或纳米级物体的组件
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摘要
Methods and compositions for detecting the assembly of complexes include providing a solution where a first portion is separated from a second portion via a submicrometer pore, submicrometer tube or channel, nanopore, or nanotube or channel. One or more submicrometer or nanometer sized object(s) is added to the first portion of the solution. Due to molecular interactions, these objects assemble to form complexes consisting of two or more submicrometer or nanometer sized objects. Passage of a complex from the first portion of the solution through the submicrometer pore, submicrometer tube or channel, nanopore, or nanotube or channel to the second portion of the solution is detected using resistive pulse sensing. This sensing methodology may comprise detecting formation of complexes in real-time and/or may comprise detecting preassembled complexes.
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