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CATALYTICALLY INACTIVE PROTEINS AND METHOD FOR RECOVERY OF ENZYMES FROM PLANT-DERIVED MATERIALS
CATALYTICALLY INACTIVE PROTEINS AND METHOD FOR RECOVERY OF ENZYMES FROM PLANT-DERIVED MATERIALS
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机译:从植物来源的材料中回收酶的非活性蛋白和酶的方法
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摘要
An inactive xylanase molecule for the recovery of xylanase activity in plant- derived material containing active xylanase enzyme(s) and xylanase inhibitors. The inactive xylanase molecule of binds to xylanase inhibitors in the plant-derived material, thereby allowing accurate measurement of xylanase enzyme activity of the enzyme contained in the plant-derived material. The invention further includes amino acid molecules depicted by SEQ ID NOS. 4 through 112, wherein the catalytically active sites of each of the amino acids have been modified resulting in inactive xylanase molecules. A method of production of the inactive xylanase molecules includes expression of the inactive xylanase molecule in microbial or eukaryal (e.g., yeast including Pichia pastoris) host cell an expression cassette comprising a promoter operably linked to a nucleic acid molecule encoding the inactive xylanase molecule and using the expressed molecule in an assay to recover the xylanase enzyme activity in plant-derived material, for example, in plant-derived material such as animal feed.
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机译:用于在含有活性木聚糖酶和木聚糖酶抑制剂的植物衍生材料中恢复木聚糖酶活性的无活性木聚糖酶分子。失活的木聚糖酶分子与植物来源的材料中的木聚糖酶抑制剂结合,从而允许精确测量植物来源的材料中所含酶的木聚糖酶活性。本发明进一步包括SEQ ID NOS所示的氨基酸分子。参照图4至112,其中每个氨基酸的催化活性位点已被修饰,导致木聚糖酶分子失活。产生非活性木聚糖酶分子的方法包括:在微生物或真核(例如,包括毕赤酵母的酵母)宿主细胞中表达非活性木聚糖酶分子;表达盒,其包含与编码该非活性木聚糖酶分子的核酸分子可操作地连接的启动子。在恢复植物来源的材料中,例如植物来源的材料(例如动物饲料)中木聚糖酶活性的试验中,分析表达的分子。
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