METHOD FOR EXTRACTING A PLATELET AGGREGATION INHIBITOR FROM AGKISTRODON BLOMHOFFII USSURIENSIS VENOM

摘要

The invention relates to producing bioactive substances and can be used for biotechnological production processes, in medicine and in the pharmacological industry, in particular to producing medicinal and diagnosis preparations from snake venom. The inventive method consists in carrying affinity chromatography on a column with a Blue Sepharose FF carrier using a Tris HCl with a PH of 7.4 in the form of a buffer solution, in diluting 8 times a material fraction unconjugated with Blue Sepharose in the Tris HCl buffer with a pH of 8.2 and in applying it to a column which is filled with a DEAE Sepharose FF anion exchanger and is pre-balanced by said Tris HCl buffer, in eluting a platelet aggregation inhibitor in the Tris HCl buffer containing NaCl, in collecting and concentrating the protein peak containing the platelet aggregation inhibitor and, with a view to substitute a buffer, in applying said peak to a column with Sephadex G25, which is balanced with the NaCl-containing HCl buffer, and in drying it by lyophilising.