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DETECTION OF WHEAT AND BARLEY FUNGAL PATHOGENS WHICH ARE RESISTANT TO CERTAIN FUNGICIDES USING THE POLYMERASE CHAIN REACTION
DETECTION OF WHEAT AND BARLEY FUNGAL PATHOGENS WHICH ARE RESISTANT TO CERTAIN FUNGICIDES USING THE POLYMERASE CHAIN REACTION
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机译:聚合酶链反应检测对某些杀菌剂具有抗性的小麦和大麦真菌病原体
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摘要
Primers specific for races of pathogenic fungi which are resistant to certain fungicides are used in polymerase chain reaction assays for the detection of fungal pathogens. The use of these primers enables the detection of specific isolates of fungal pathogens and the monitoring of disease development in plant populations. The invention includes DNA sequences which show variability between different fungal pathotypes. Such DNA sequences are useful in the method of the invention as they can be used to derive primers for use in polymerase chain reaction (PCR)-based diagnostic assays. These primers generate unique fragments in PCR reactions in which the DNA template is provided by specific fungal pathotypes and can thus be used to identify the presence or absence of specific pathotypes in host plant material before the onset of disease symptoms.
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