Method for collecting cells in M phase or G1 phase and method for nuclear transplantation using the cells

摘要

Methods for collecting cells in M phase or G₁ phase, by which the percentage of M phase or G₁ phase cells is higher than that attained by the conventional methods are disclosed. The method for collecting cells in M phase comprising, in the order mentioned, the steps of:(1) culturing mammalian cells on a support in the presence of an agent for synchronizing cell cycle to metaphase;(2) shaking the support and recovering cells detached from the support; and(3) recovering cells having a diameter of not less than 20 µm from the cells recovered in the step (2).