There is provided a Rhodobacter sphaeroides SK2H2 (Accession No.: KCTC10915BP) strain having a high content of coenzyme QlO, and a method of producing coenzyme QlO using the same, the method including culturing the Rhodobacter sphaeroides SK2H2 (Accession No.: KCTC10915BP) strain having a high content of coenzyme QlO and isolating coenzyme QlO from a culture broth of the strain. The Rhodobacter sphaeroides SK2H2 (Accession No.: KCTC10915BP) strain has a very high content of coenzyme QlO per dry cell weight, which is greater than 20 mg/gDCW, compared to the previously produced strains, and therefore it is possible to produce coenzyme QlO effectively in a large scale culture of the strain.
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