METHOD OF DETECTING GENE METHYLATION AND METHOD OF EXAMINING NEOPLASM BY DETECTING METHYLATION

摘要

An object of the present invention is to provide a method for detecting methylation in genes contained in a biological sample in a simple and economical manner. Further object of the present invention is to provide an accurate and good sensitivity examination method for a case the neoplasm is assayed using method for detecting methylation in DNAs. The biological sample is supplied with a polysaccharide before it is processed with a bisulfite in order to detect methylation in genes, allowing processing with a bisulfite thereafter, and thereby to allow detection of methylated cytosine which has not been modified to convert into uracil, even if the sample is not supplied with protein denaturing agent and the DNA is not purified to detect. Further, the present invention can examine neoplasm more accurately, because it detects methylation on a plurality of genes contained in the biological sample to calculate the total of their respective methylation values.