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METHOD OF BREEDING CELLS HAVING IMPROVED TOLERANCE TO SHORT-CHAIN FATTY ACIDS
METHOD OF BREEDING CELLS HAVING IMPROVED TOLERANCE TO SHORT-CHAIN FATTY ACIDS
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机译:提炼对短链脂肪酸具有耐受性的细胞的方法
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摘要
It is intended to provide a method of conferring to microbial cells or the like, tolerance to a short chain fatty acid, including formic acid and acetic acid, which is harmful to the growth thereof without suppressing the growth of the cells while maintaining a high productivity of useful substances. It is also intended to provide a means for efficiently culturing a microorganism in the presence of a short chain fatty acid, or a means for producing a useful short chain fatty acid via fermentation.;The present invention provides a method of breeding cells to improve tolerance to a short chain fatty acid, wherein a gene encoding a protein having a function of transporting a short chain fatty acid from the inside of cells to the outside of cells, is transformed into and expressed in the cells; a high cell-density culture method for producing useful substances using the cell; and a method of preparing a fermentation broth comprising a short chain fatty acid, wherein the cells are cultured under the conditions such that the cells produce a short chain fatty acid.
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