METHOD OF ASSAYING PROTEIN IN SAMPLE AND ASSAY REAGENT THEREFOR

摘要

It is intended to provide a method of highly sensitively assaying a protein in a sample which can be conducted at a low cost, is applicable not only to an apparatus for exclusive use but also to automatic analyzers of common type that have been employed in a large number of hospitals, and enables the assay of even a protein contained in a minor amount in a sample; and an assay reagent therefor. The above-described objects can be achieved by the following method of assaying a protein in a sample and an assay reagent therefor. Namely, the above-described method of assaying a protein in a sample comprises bringing the sample into contact with an anionic compound having a fluorescein skeleton and a surfactant, and measuring the absorbance of the liquid mixture containing the sample, the anionic compound having a fluorescein skeleton and the surfactant. The reagent for assaying a protein in a sample as described above contains an anionic compound having a fluorescein skeleton and a surfactant.