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Method for detecting a target molecule employing at least two affinity probes and rolling circle replication of padlock probes
Method for detecting a target molecule employing at least two affinity probes and rolling circle replication of padlock probes
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机译:使用至少两种亲和探针和挂锁探针的滚环复制检测靶分子的方法
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摘要
Rolling circle replication of a padlock primer is inhibited when it is hybridised to a target nucleic acid that is long or circular. The invention provides methods of addressing this problem including cutting the target nucleic acid near or preferably at the site which hybridises with the padlock probe, whereby a 3'-end of the cut target nucleic acid acts as a primer for rolling circle replication of the padlock probe. Also included is a method for assaying for a polyepitopic target by the use of two affinity probes each carrying an oligonucleotide tag and of padlock probe for rolling circle replication in association with the two affinity probes.
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