ROLLING CIRCLE REPLICATION OF PADLOCK PROBE

摘要

PROBLEM TO BE SOLVED: To provide a method of rolling circle replication of a padlock primer when the padlock primer is hybridized to a target nucleic acid that is long or circular.SOLUTION: The methods for assaying for a polyepitopic target by the use of two affinity probes each carrying an oligonucleotide tag and of padlock probe for rolling circle replication in association with the two affinity probes includes cutting the target nucleic acid near or preferably at the site which hybridizes with the padlock probe, whereby a 3'-end of the cut target nucleic acid acts as a primer for rolling circle replication of the padlock probe.