COSMETIC OR PLASTIC COMPOSITION COMPRISING MULTIPOTENT STEM CELLS DERIVED FROM HUMAN ADIPOSE TISSUE, FIBROBLAST AND ADIPOSE OR ADIOPOCYTE

摘要

A composition comprising human adipose tissue-derived adult stem cells, fibroblasts and adipose or adipocytes is provided to be useful for cosmetic purposes such as skin tightness improvement, wrinkle and flabby skin improvement, and depressed area restoration through plastic surgery as well as useful for the formation of new breast tissue due to the adult stem cells capable of being differentiated into fat cells. A composition for cosmetic or plastic surgery comprises human adipose tissue-derived adult stem cells, fibroblasts and adipose or adipocytes. A method for preparing the composition comprises the steps of: (a) culturing an adipose-containing suspension obtained from human adipose tissue by liposuction in a culture flask and then collecting cell layers comprising both adipose-derived adult stem cells and fibroblasts, which are attached on the surface of the culture flask; and (b) mixing the collected cell layers with adipose or adipocytes, wherein the adult stem cells are mesoderm-derived cells and the adipose-derived adult stem cells are cultured in an alpha-MEM medium including dexamethasone, indomethacin, insulin and IBMX to differentiate into adipogenic cells.