Vector for evaluating the activity of promoter evaluating method using the same and promoter isolated by the method

摘要

A vector is provided to measure the promoter activity accurately by conveniently correcting errors of the promoter activity measurement result in accordance with the transformation efficiency of a cell without going through a complex process. A vector for measuring the promoter activity is characterized in that a CMV promoter, a red fluorescent protein gene, a poly A signal sequence, a multi-cloning site, and a green fluorescent protein gene and a poly A signal sequence are linked in sequence and it includes a replication origin and an antibiotic resistant gene. A method for measuring the promoter activity comprises the steps of: (a) inserting a candidate DNA fragment into the multi-cloning site of the vector; (b) introducing the vector into a cell to transform it; (c) measuring the amount of the fluorescence of the transformed cell to select the red fluorescent protein gene-expressed cells; and (d) measuring the expression level of the green fluorescent protein gene from the selected cells and then comparing it with the expression level of the red fluorescent protein gene.