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PROCESS AND GENES FOR EXPRESSION AND OVER-EXPRESSION OF ACTIVE FEFE HYDROGENASES
PROCESS AND GENES FOR EXPRESSION AND OVER-EXPRESSION OF ACTIVE FEFE HYDROGENASES
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机译:活性[FEFE]加氢酶表达和过表达的过程和基因
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摘要
A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H2 production.
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