METHOD OF DESIGN PROBES FOR DETECTING TARGET SEQUENCE AND METHOD OF DETECTING TARGET SEQUENCE USING THE PROBES

摘要

The present invention provides a kind of method and the detection for detecting target sequence using the method designed for detection target sequence. Probe design process according to the present invention for detecting target sequence includes the steps that selecting position in a) target sequence to be detected and with the sequence homology of the non-targeted sequence of highest different from target sequence as reference position; B) selection includes the steps that the first probe design section in the target sequence of predetermined A includes the ReA of reference position, and all probes all have certain length, which is designed in the region of reference position; C) the step of position in selection target sequence is different from non-targeted sequence to probe selection position; D) include the steps that probe selects position to the positions th. The centers E and exact matching probe (hybridization) n for hybridizing completely of selection) possible sequence and target sequence; E) probe than exactly matching is long, the first includes positioned at the probe selection position of a position, the position includes the nucleotide that can not hybridize with the target sequence for being located at the second position, the target sequences of all positions other than the second position and selection are by expected the step of mismatching probe of the Sequence composition, hybridization sequences as fully as possible.