首页> 外国专利> EXPRESSION VECTOR HAVING PROMOTER SEQUENCE OF VASA HOMOLOGUE GENE DERIVED FROM MAMMAL AND USE THEREOF

EXPRESSION VECTOR HAVING PROMOTER SEQUENCE OF VASA HOMOLOGUE GENE DERIVED FROM MAMMAL AND USE THEREOF

机译:具有哺乳动物源VASA同源基因启动子序列的表达载体及其应用。

摘要

Disclosed is a method for screening a cell capable of differentiating into a germ cell in a simple manner by utilizing the expression of a marker gene as an indicator, which does not need any complicated operations such as homologous recombination. A method for producing a germ cell comprising the step of collecting a cell capable of differentiating into a germ cell from a transgenic non-human mammal having a recombinant expression vector introduced therein utilizing the expression of a marker gene as an indicator, the recombinant expression vector having the marker gene integrated therein such a manner that the marker gene is placed under the regulation of a promoter sequence of a Vasa homologue gene derived from a mammal.
机译:公开了一种通过利用标志物基因的表达作为指示物以简单的方式筛选能够分化为生殖细胞的细胞的方法,该方法不需要任何复杂的操作,例如同源重组。一种生产生殖细胞的方法,包括以下步骤:收集能够从其中引入有重组表达载体的转基因非人类哺乳动物利用标记基因的表达作为指示剂分化为生殖细胞的细胞,该重组表达载体具有整合在其中的标记基因的方式,使得将标记基因置于源自哺乳动物的Vasa同源基因的启动子序列的调控下。

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