MASS PRODUCTION OF BULBLET VIA SOMATIC EMBRYOGENIC CELL CULTURE IN LILY

摘要

Bulbil lily (lily sp. ) a production method by rapidly spread body blastocyte be arranged to produce a large amount of lily bulbils in a short time, by using a method for tissue culture of lily scale and a bioreactor culture technology, so that the viral free stock of lily bulbil is produced in enormous quantities. One production method of liliaceous bulbil includes the following steps:: (1) the MS of a solid (Murashige Skoog) it is moderate include 4- amino -3,5, lily scale or 3 is cultivated in 6- trichloropicolinic acids, 6 two chloro- O- anisic acids are in pH 5.6-5.The blastocyte of 8 and 25 DEG C of induction bodies under dark condition; (2), in pH 5. under dark condition6-5.8 and the 20-25 DEG C of blastocyte in no hormone MS media culture body, induce the obesity of lily bulbil, chinesization is executed at the dissolved oxygen concentration of 40 ppm and the agitation speed of 100 rev/min.