MEDIUM AND METHOD FOR CELL SUSPENSION CULTURE OF RHODILOA SACHALINENSIS, AND FEED ADDITIVES AND FEEDS CONTAINING RHODILOA SACHALINENSIS CELLS OBTAINED FROM THE SAME

摘要

A medium for cell suspension culture of Rhodiola sachalinensis is provided to increase the content of salidroside in cultured Rhodiola sachalinensis as compared with wild Rhodiola sachalinensis, and improve production yield of Rhodiola sachalinensis, so that the cultured Rhodiola sachalinensisis useful as feed additive and feed. A medium for cell suspension culture of Rhodiola sachalinensis comprises 0.5-1 mg/l of naphthalene acetic acid and 2-5 mg/l of N6-benzyladenine in B5(2B5) medium containing 95-100 mM of KNO3, 0.8-2 mM of (NH4)2SO4, 2-4 mM of NaH2PO4.2H2O, 1-6 mM of CaCl2.2H2O and 1-6 mM of MgSO4.7H2O, and further comprises at least one selected from 0.001-0.05 mg/l of thidiazuron, 1-2 mg/l of zeatin, 1-50 mg/l of spermine and 1-100mg/l of spermidine and is free of gibberellic acid. The Rhodiola sachalinensis callus is suspension-cultured in the medium at aeration rate of 0.3-0.5 l/min for 4-5 weeks.