SALMONELLA MUTANTS DELETED LON GENE AND/OR CPXR GENE AND LIVE SALMONELLA VACCINES COMPRISING THEREOF

摘要

An attenuated Salmonella live vaccine is provided to be easily and economically produced and stored and prevent Salmonella infection safely and efficiently when administered to a domestic animal. A method for preparing a Salmonella live vaccine comprises a step of deleting a lon or a cpxR gene from a chromosome DNA of a Salmonella strain such as Salmonella typhimurium, S. typhi, S. paratyphi, S. sendai, S. gallinarium and S. enteritidis. A vector includes a DNA where a DNA fragment linked to a 5'-terminal of the lon gene derived from the Salmonella strain and a DNA fragment linked to a 3'-terminal of the lon gene are bonded. An attenuated Salmonella vaccine composition comprises a Salmonella mutant where the lon gene and the cpxR gene are deleted from the chromosome DNA of the Salmonella and a pharmaceutically acceptable carrier.