A Novel in vivo Perfusion Method for inducing Differential Signaling in An Animal

摘要

The present invention relates to a novel in vivo perfusion method capable of obtaining a large amount of proteins together with the most ideal control protein sample, wherein the proteins changed specifically to cell signaling are modified into the most similar form to be present under in vivo conditions. More particularly, the present invention relates to a method for facilitating researches on diseases caused by abnormal cell signaling, or screenings of various drugs and in vivo ligands in the similar form to that present under in vivo conditions. Also, this is an innovative method capable of solving the problems, for example the small amount of produced sample and the controls in its production, in the studies on the direct changes in tissues or cells by various compounds (for example, cell growth factors or medical supplies) which are administered to elucidate a cell signaling mechanism and a physiological phenomenon.